有关EMSA实验中的各种问题及解决方案(与EMSA基本知识无关)全部完成了。遇到问题的客户,应可以从中找到答案。本英文及附属网页原是美国微奥基因网站内容,因为我们以前的原创文案常被别的公司盗版,我们就不准备翻译成中文了,如有任何问题,我们乐意解答(当然是中文解答),谢谢理解。
| Methods | Problems /Solution | Major procedures | 微奥基因's products | 
|---|---|---|---|
| Pre-EMSA | Well design is a good start | Experimental design | Help customers for their studies | 
| What needs for EMSA | Equipments & reagents | Devices, kit & reagents | |
| Bad samples cause a major EMSA failure | Sample preparation | 
								Nuclear protein prep. Nuc. pro. concentration Positive controls. Sample Prep. service  | 
							|
| High quality probes are nescessary for EMSA | Probe preparation | 
								
								Ready biotin-probes Ready IR Fluo-probes Custom EMSA probes  | 
							|
| 
								Biotin/ECL EMSA  | 
								See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers  | 
							
| ECL EMSA-related problems and their solution. | 
								Protein-probe reaction Running gels Transblots UV crosslink Biotin/Avidin/HRP reaction ECL Imaging  | 
								
								Complete EMSA kits General EMSA kits EMSA reagents ECL substrates ECL imagers EMSA controls EMSA Services  | 
							|
| 
								IR 
								Fluo- EMSA  | 
							|||
| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers  | 
							|
| IR EMSA-related problems and their solution. | 
								Protein-probe reaction Running gels Imaging gels  | 
								
								Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents EMSA controls EMSA Services  | 
							|
| 
								Gel-Stain EMSA  | 
							|||
| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers  | 
							|
| Gel-stain EMSA-related problems and their solution. | 
								Protein-probe reaction Running gels Staining gels Imaging gels  | 
								
								
								Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services  | 
							|
| 
								Competitive EMSA  | 
							|||
| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers  | 
							|
| Competitive(竞争) EMSA-related problems and their solution. | 
								Protein-probe reaction Running gels Staining gels Imaging gels  | 
								
								
								Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services  | 
							|
| 
								Supershift EMSA  | 
								See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers  | 
							
| Supershift (超迁移)EMSA-related problems and their solution. | 
								Protein-probe reaction The rest is same as ECL or IR EMSA  | 
								
								Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services  | 
							|
| RNA EMSA | |||
| See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers  | 
							|
| RNA EMSA-related problems and their solution. | 
								Protein-probe reaction The rest is same as ECL or IR EMSA  | 
								
								Custom probes General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Service  |