有关EMSA实验中的各种问题及解决方案(与EMSA基本知识无关)全部完成了。遇到问题的客户,应可以从中找到答案。本英文及附属网页原是美国微奥基因网站内容,因为我们以前的原创文案常被别的公司盗版,我们就不准备翻译成中文了,如有任何问题,我们乐意解答(当然是中文解答),谢谢理解。
Methods | Problems /Solution | Major procedures | 微奥基因's products |
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Pre-EMSA | Well design is a good start | Experimental design | Help customers for their studies |
What needs for EMSA | Equipments & reagents | Devices, kit & reagents | |
Bad samples cause a major EMSA failure | Sample preparation |
Nuclear protein prep. Nuc. pro. concentration Positive controls. Sample Prep. service |
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High quality probes are nescessary for EMSA | Probe preparation |
Ready biotin-probes Ready IR Fluo-probes Custom EMSA probes |
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Biotin/ECL EMSA |
See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
ECL EMSA-related problems and their solution. |
Protein-probe reaction Running gels Transblots UV crosslink Biotin/Avidin/HRP reaction ECL Imaging |
Complete EMSA kits General EMSA kits EMSA reagents ECL substrates ECL imagers EMSA controls EMSA Services |
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IR
Fluo- EMSA |
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See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
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IR EMSA-related problems and their solution. |
Protein-probe reaction Running gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents EMSA controls EMSA Services |
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Gel-Stain EMSA |
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See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
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Gel-stain EMSA-related problems and their solution. |
Protein-probe reaction Running gels Staining gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
|
Competitive EMSA |
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See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
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Competitive(竞争) EMSA-related problems and their solution. |
Protein-probe reaction Running gels Staining gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
|
Supershift EMSA |
See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
Supershift (超迁移)EMSA-related problems and their solution. |
Protein-probe reaction The rest is same as ECL or IR EMSA |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
|
RNA EMSA | |||
See how to make high quality gels | Casting gels | Precasting gels Gel-making sol. Running buffers |
|
RNA EMSA-related problems and their solution. |
Protein-probe reaction The rest is same as ECL or IR EMSA |
Custom probes General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Service |