荧光/化学光成像仪

  

【Cool Imager™】- 高灵敏生物发光与荧光数字成像仪 (IMGR002, IMGR003)

一、产品背景常规的生物图像与生物信号用肉眼观察灵敏度低或根本无法进行肉眼观察。常常需要采用酶显色或放射性同位素放大后进行观察。近年兴起的Chemiluminescence(化学发光)因其极高灵敏度与优越的线性关系得到普遍应用。用光学照相机照像或胶片显影法显示结果则需要暗室以及胶片,照相纸,冲洗液等耗材。图像的定量分析与后期处理也较困难。相比之下,通过数字相机的数字成像技术则有许多优点。国内市场上出售的电泳图像成像系统因灵敏度低而不能用于显示化学自发光反应 (Chemiluminescence)以及荧光示踪。结合现代生物学,光学,半导体及计算机技术,我公司开发了高灵敏度的数字成像系统(Cool Imager)。 Cool Imager为多用途数字成像及图像分析工作站,主要用于酶催化的化 学自发光反应与荧光图谱。由于对摄像系统采用了深低温冷却(-45oC)技术,灵敏度较传统的酶底物显色法提高约2000倍。

完全成像系统包括所有硬件、并已经安装系统与分析软件,开箱后即可使用。高度集成的系统已经包括微机,不需要另配计算机。系统已经优化。系统启动与关闭迅速。采像单元可每分钟采积并累加近2000幅图。最高累加图幅达200,000幅图,采像时间可达25分钟。一般取图5,000幅左右即可在计算机屏幕清晰显示蛋白印迹图或分子杂交图。由于采图数是任意可变的,Cool Imager可扩展用于摄取DNA/RNA凝胶图,考马氏亮兰及银染的蛋白凝胶图,以及蛋白荧光图谱。

本工作台可连接显微摄像头摄取彩色显微镜图像。
  

二、技术指标

系统冷却达-45oC,可以使微弱的发光成像。曝光25分钟、累集10,000幅图没有明显杂色背景。
  

三、技术参数(CoolImager II)

 

 

 

 

 

 

 

 

 

 

MAIN UNIT

Complete System

Cool Imager™ is an all-in-one unit and comes with all the hardware including the monitor, keyboard, mouse, pre-installed operating system and analytical software. After you receive the units, plug in and use; that is it. No custom hardware and software installation are required.

High Integration

The unit is integrated with the computer-board with an embedded CPU, the control board and operating panel, optical devises, a frame grabber, lights, filters and a mini darkroom chamber. This specific design increases efficiency and stability of the workstation.

Low-noise

Technology

Low-noise technology is applied to all the electronic components in the unit to prevent the interruption of electro-noise and reduce vibration, which makes the design of highly integrated system possible and enhances the stability of the unit.

Sensitivity

Detects as little as ~0.5 fg (10-17g) of HRP with Lightgen™ substrates and completely eliminates the use of X-ray film for chemiluminescent imaging.

Imaging Capacity

Cool imager can integrate ~30 frames per second and collect ~50,000 frames in ~30min, and its sensitivity is better than autoradiography using radioisotope p32 or S35.

Darkroom

The mini darkroom is light-proofed by a design with multi-staged, magnetic sealing strips, which permit the door to open and close easily.

Self-contained

Unit

There is no external camera or lens outside of the unit. All electronic and optical components are enclosed inside the unit, which provides a moisture-proof, dust-proof environment and extend the service life of the electronic and optical components.

Power

Operation

Workstation uses a flat control panel to turn on and off the operation program and also controls image capturing, light source, power lens and camera functions.

 

 

 

SOFT-Ware

Operation

Pre-installed Windows® (Microsoft) or Linux®  (Redhat) operation program

Imaging & Analyzing Software

With the software, ViaCapture, capturing an image is achieved simply by entering the number of integration. 1D gel, 2D dot-blot, bacterial colony, Western blots, DNA/RNA hybridization, EMSA and microplates can be imaged and saved by Cool Imager™. The captured images can be opened and edited with ViaAnalysis software. ViaAnalysis can invert, correct slanted images, adjust image contrast and brightness, perform image quantitation and more. All images can be copied, pasted or printed.

Other programs

Office, Image editor, PDF, Zip and Antivirus programs are pre-installed.

C C D

The ultra-cooled, C-Mount camera with 2-stage Peltier cooling mechanism, cools temperature down to -45oC and reduces the background noise down to 17 e- rms. The cooled camera has an 8 bit, ½ inch CCD installed with a pixel size of 6.4um X 6.4um. The resolution can reach up to 1642H X 1024V.

LENS

F=1:1.0, 8~48mm optical lens with power zoom, power focus and iris.

LIGHTS

A set of white reflective lights and 3 sets of colored reflective lights (Cold Light) are included. Transmitting blue, ultra-violet or white light boxes are sold separately at an additional cost.

FILTERS

A tunable filter wheel which can hold 4 sets of filters. A brown filter is included for imaging DNA/RNA gels stained with Ethidium bromide or SYBR Green. Other filters are options sold separately at an additional cost.

注; 摄像机的冷却温度与仪器灵敏度有关。温度越低,杂色越少,灵敏度越高,价格高。 VersaDoc未配置变焦镜头,没有图像放大功能,不能用于基因芯片图。 CoolImager配专门技术用于我公司推出的EMSA系列成套试剂。其他公司的仪器不能显示结果。Cool Imager本身具有所有控制单元的软,硬件。只需外接显示器即可使用。

 

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